HEPES
化合物
HEPES（4-(2-羟乙基)-1-哌嗪乙磺酸）是一种两性离子磺酸缓冲剂，它是二十种古德缓冲试剂之一，广泛用于细胞培养。[2]它和1-甲基-3-乙基咪唑𬭩形成离子液体。[3]
| HEPES | |
|---|---|
| |
| IUPAC名 2-[4-(2-Hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid | |
| 别名 | HEPES |
| 识别 | |
| CAS号 | 7365-45-9 
|
| PubChem | 23831 |
| ChemSpider | 22278 |
| SMILES |
|
| InChI |
|
| InChIKey | JKMHFZQWWAIEOD-UHFFFAOYAC |
| Beilstein | 883043 |
| EINECS | 230-907-9 |
| ChEBI | 42334 |
| RTECS | TL6809000 |
| 性质 | |
| 化学式 | C8H18N2O4S |
| 摩尔质量 | 238.3 g·mol−1 |
| 外观 | white crystalline powder |
| 密度 | Not applicable |
| 熔点 | >234-238°C (453-457K) |
| 溶解性（水） | 40 g/100 ml (20°C) |
| pKa | 3 (pKa1), 7.5 (pKa2)[1] |
| 危险性 | |
GHS危险性符号
| |
| GHS提示词 | 警告 |
| H-术语 | H315, H319, H335 |
| P-术语 | P261, P264, P270, P271, P280, P301+312, P302+352, P304+312, P304+340, P305+351+338, P312, P321, P322, P330 |
| 主要危害 | Eye Irritant |
| NFPA 704 |
 0
1
1
|
| 闪点 | Non-flammable |
| 若非注明，所有数据均出自标准状态（25 ℃，100 kPa）下。 | |
参考文献
- ^ Johnson MA, Seifert S, Petrache HI, Kimble-Hill AC. Phase Coexistence in Single-Lipid Membranes Induced by Buffering Agents. Langmuir. 2014, 30 (33): 9880–9885. PMC 4148158
. doi:10.1021/la5018938.
- ^ Baicu SC, Taylor MJ. Acid-base buffering in organ preservation solutions as a function of temperature: new parameters for comparing buffer capacity and efficiency. Cryobiology. 2002, 45 (1): 33–48. PMID 12445548. doi:10.1016/S0011-2240(02)00104-9.
- ^ Mohamed Taha; et al. Good's buffers as a basis for developing self-buffering and biocompatible ionic liquids for biological research. Green Chem., 2014,16, 3149-3159. doi:10.1039/C4GC00328D.
